Fig. (3) A,B,C. Analysis of emotionality- and anxiety-related behaviour in the open field test of male and female control rats, handled (HD) rats, and maternal deprived (MD) rats. (A) Female and male HD and MD rats demonstrated decreased locomotor activity compared to control rats in the novel environment, but the difference was significant in females, only (p = 0.001). (B) Female and male MD rats as well female HD rats tended to spent less time in in the illuminated anxiety-related centre field of the open field apparatus (NS). (C) HD and MD rats reared less than controls, but this behavioural effects was significant in females, only (p < 0.001).