Fig. (3) Total FITC-HA binding to CD14+ PBMC for each dosing period.The binding of FITC-HA to CD14+ PBMC (monocytes) was quantified by FACS and total binding was calculated by measuring AUC as described in Subjects, Materials and Methods. Values represent the mean of samples from 6 volunteers ± SE. AUC was significantly greater in the 4^th dosing period when compared to periods 1-3 (P<0.0001).