Fig. (6) Real time PCR determination of RNA expression levels in drug naïve parental DU-145 cells (Parent) and drug resistant DU-145 cells (Resistant) indicates that acquisition of resistance to AG2034 is associated with increases in ERK1/2, MEK1/2, B-Raf and N-Ras. Total RNA was isolated after 48 h of plating cells in fresh 0HPX media continuously supplemented with (Resistant cells) or without (Parental cells) 50 nM AG2034. Panel A: ERK1/2; Panel B: MEK1/2; and Panel C: N-Ras and B-Raf. Data denote means ± S.D. of three independent determinations.