Fig. (9) The effect of silencing ERK1/2 on GARFT mRNA and protein expression levels in parental and drug-resistant DU145 cells. Parental or drug resistant cells (1 x 10⁴ per well) were plated in the absence or presence respectively, of 50 nM AG2034. After allowing 24 h to adhere, cells were transfected with ERK1/2 specific or non-specific siRNA probes. Resistant cells were continually maintained in the presence of drug. At hour 4, 1 ml of fresh media was added to each well. Transient transfections lasted for a total of 34 h after which total RNA and protein were extracted. Data in panel A represent means ± S.D. of three independent determinations. The results in Panel B are representative of two individual blots.