Fig. (3) Contour plots for temperature versus stirring speed in the hibiscus calyces extraction for TMA (Y2) (A), where the variable X1 was fixed in 0.5; and for ABTS (Y3) (B), where the variables X1 and X4 were fixed in 0.5 (X1: enzyme concentration; X2: temperature; X3: stirring speed; X4: time).