Table 2: Experimental design and determination of Minimum Inhibitory Concentration and Minimum Bactericidal Concentration for the bacteria tested.
| – |
VARIABLES |
Gram-positive |
Gram-negative |
| Extract |
M (g) |
E (%) |
T (°C) |
S. aureus (μg.mL-1) |
S. Typhimurium (μg.mL-1) |
P. aeruginosa (μg.mL-1) |
E. coli (μg.mL-1) |
| 1 |
4.02 |
25.13 |
38.00 |
39.00 |
42.00 |
- |
42.00 |
| 2 |
4.02 |
25.13 |
62.00 |
- |
- |
- |
- |
| 3 |
4.02 |
54.87 |
38.00 |
32.00 |
- |
35.00 |
- |
| 4 |
4.02 |
54.87 |
62.00 |
29.00 |
37.00 |
37.00 |
37.00 |
| 5 |
8.48 |
25.13 |
38.00 |
70.00 |
30.00 |
60.00 |
60.00 |
| 6 |
8.48 |
25.13 |
62.00 |
60.00 |
59.00 |
59.00 |
59.00 |
| 7 |
8.48 |
54.87 |
38.00 |
78.00 |
- |
83.00 |
83.00 |
| 8 |
8.48 |
54.87 |
62.00 |
63.00 |
64.00 |
32.00 |
- |
| 9 |
2.50 |
40.00 |
50.00 |
21.00 |
- |
- |
- |
| 10 |
10.00 |
40.00 |
50.00 |
96.00 |
55.50 |
55.50 |
111.00 |
| 11 |
6.25 |
15.00 |
50.00 |
40.00 |
36.00 |
- |
- |
| 12 |
6.25 |
65.00 |
50.00 |
29.00 |
- |
35.00 |
- |
| 13 |
6.25 |
40.00 |
30.00 |
43.00 |
49.00 |
49.00 |
49.00 |
| 14 |
6.25 |
40.00 |
70.00 |
40.00 |
44.00 |
- |
44.00 |
| 15 |
6.25 |
40.00 |
50.00 |
51.00 |
50.00 |
50.00 |
50.00 |
M- mass; E-ethanol; T - Temperature.
-: there was no inhibition at the highest concentrations evaluated.