| Sample number | Sample site | FA result | Culture result | Interpretation | |
|---|---|---|---|---|---|
| FA target detected, but not confirmed on culture (n=7) |
11 | Pus (finger) |
S. aureus, S. agalactiae, E. coli |
E. coli, Anaerobic Gram negative cocci |
E. coli detected on FA and culture Anaerobic GNC not on panel Potentially non-viable S. aureus and S. agalactiae therefore not cultured |
| 21 | Joint fluid | Streptococcus species | No growth | Potentially non-viable Streptococcal species in sample or may represent presence at low levels in sample, not consistent with infection | |
| 30 | Pleural fluid | S. pyogenes | No growth | Potentially non-viable S. pyogenes DNA in cultured sample | |
| 52 | Pus (neck) | S. pyogenes | No growth | Potentially non-viable S. pyogenes DNA in cultured sample | |
| 56 | Pus (submental abscess) |
S. pyogenes H. influenzae |
S. pyogenes | True positive S. pyogenes Potentially missed H. influenzae on culture |
|
| 139 | Tissue (hand) | Pseudomonas aeruginosa | No growth | Potentially non-viable bacteria in sample or present in low levels without causing infection due to site of collection (hand) | |
| 146 | Pus (sinus) | S. pyogenes | S. epidermidis |
Non-viable S. pyogenes DNA in cultured sample Question significance of S. epidermidis on cultured specimen, possible contaminant |
|
| Missed organisms (n=9) |
7 | PDFL | No target detected |
E. coli Moraxella species |
Missed E. coli Moraxella not on FA panel |
| 12 | Pus (perinasal) | H. influenzae |
C. albicans S. dysgalactiae Aggegatibacter species Bacteroides fragilis |
False positive H. influenzae detection (potential misidentification as Aggregatibacter) Missed C. albicans and Streptococcal species Aggregatibacter and Bacteroides not on panel |
|
| 20 | Joint fluid | No target detected | S. pyogenes | Missed organism | |
| 59 | Joint fluid | No target detected | S. aureus | Missed organism | |
| 65 | Fluid (intra-abdominal) | No target detected |
S. anginosus B. fragilis |
Missed Streptococcal species B. fragilis not on panel |
|
| 70 | Fluid (intra-abdominal) | No target detected |
S. anginosus B. fragilis |
Missed Streptococcal species B. fragilis not on panel |
|
| 103 | Fluid (Pseudomeningocoele) | No target detected |
E. coli S. constellatus |
Missed organisms | |
| 115 | Fluid (intra-abdominal) | No target detected |
E. coli S. constellatus |
Missed organisms | |
| 136 | Fluid (pump device) | No target detected | S. aureus | Missed organism | |
| Organisms cultured which did not have targets on FA panel (n=8) |
7 | PDFL | No target detected |
E. coli Moraxella species |
Missed E. coli Moraxella not on FA panel |
| 11 | Pus (finger) |
S. aureus S. agalactiae E. coli |
E. coli Anaerobic Gram negative cocci |
E. coli detected on FA and culture Anaerobic GNC not on panel Potentially non-viable S. aureus and S. agalactiae therefore not cultured |
|
| 12 | Pus (perinasal) | H. influenzae |
C. albicans S. dysgalactiae Aggegatibacter species Bacteroides fragilis |
False positive H. influenzae detection Missed C. albicans and Streptococcal species Aggregatibacter and Bacteroides not on panel |
|
| 29 | Fluid (occipital aspirate) | No target detected | Aspergillus fumigatus | Organism not on FA panel | |
| 65 | Fluid (intra-abdominal) | No target detected |
S. anginosus B. fragilis |
Missed Streptococcal species B. fragilis not on panel |
|
| 70 | Fluid (intra-abdominal) | No target detected |
S. anginosus B. fragilis |
Missed Streptococcal species B. fragilis not on panel |
|
| 123 | Pus (abdominal fluid) | Streptococcal species detected |
S. intermedius Eikenella corrodens |
Correct identification of Streptococcal species E. corrodens not on FA panel |
|
| 144 | Pus (mastoid abscess) | Streptococcal species |
S. constellatus Moraxella catarrhalis |
Correct identification of Streptococcal species M. catarrhalis not on FA panel |