|
Batch n°/ Sample n° |
Sampling Date | Mouse Bioassay (MBA)1 + Seroneutralisation (SN) and Enrichment followed by qPCR | Result without/with Seroneutralisation (SN)2 | Conclusion |
|---|---|---|---|---|
| 1/ Serum 1 | 15-March | BoNT (MBA) + SN ABE-F | † / - | BoNT detected |
| 1/ Serum 2 | 20-March | BoNT (MBA) + SN B | †/ - | BoNT B detected |
| 1/ Serum 3 | 23-March | BoNT (MBA) + SN B | † / - | BoNT B detected |
| 1/ Faeces 1 | 23-March | BoNT (MBA) + SN ABE-F | † / † (non specific) | BoNT Not detected |
| C. botulinum (MBA after enrichment) | negative | BoNT Not detected | ||
| BoNT gene (qPCR) | + | C. botulinum type B | ||
| 2/ Ham (small) | 26-March | BoNT (MBA with trypsin treatment) | † | BoNT detected |
| C. botulinum (MBA after enrichment) + SN B | †/ - | BoNT B producing-C. botulinum | ||
| BoNT gene (qPCR) | + | C. botulinum type B | ||
| 2/ Ham (big) | 27-March | BoNT (MBA with trypsin treatment) | † | BoNT detected |
| C. botulinum (MBA after enrichment) + SN B | †/ - | BoNT B producing-C. botulinum | ||
| BoNT gene (qPCR) | + | C. botulinum type B | ||
| 3/ Serum 4 | 30-March | BoNT (MBA) + SN B | specific mild symptoms 1, 2, 3 | BoNT B detected |
| 3/ Faeces 2 | 31-March | BoNT (MBA) | - | BoNT Not detected |
| C. botulinum (MBA after enrichment) | - | BoNT Not detected | ||
| BoNT gene (qPCR) | - | BoNT Not detected | ||
| 4/ Serum 5 | 6-Apr | BoNT (MBA) + SN B | specific mild symptoms 1, 2, 3 | BoNT B detected |
| 4/ Faeces 3 | 7-Apr | BoNT (MBA) | - | BoNT Not detected |
| C. botulinum (MBA after enrichment) | - | BoNT Not detected | ||
| BoNT gene (qPCR) | - | BoNT Not detected | ||
| 5/ Serum 6 | 21-Apr | BoNT (MBA) | - | BoNT Not detected |