Table 1: Results of investigated food and human samples.

Batch n°/
Sample n°
Sampling Date Mouse Bioassay (MBA)1 + Seroneutralisation (SN) and Enrichment followed by qPCR Result without/with Seroneutralisation (SN)2 Conclusion
1/ Serum 1 15-March BoNT (MBA) + SN ABE-F † / - BoNT detected
1/ Serum 2 20-March BoNT (MBA) + SN B †/ - BoNT B detected
1/ Serum 3 23-March BoNT (MBA) + SN B † / - BoNT B detected
1/ Faeces 1 23-March BoNT (MBA) + SN ABE-F † / † (non specific) BoNT Not detected
C. botulinum (MBA after enrichment) negative BoNT Not detected
BoNT gene (qPCR) + C. botulinum type B
2/ Ham (small) 26-March BoNT (MBA with trypsin treatment) BoNT detected
C. botulinum (MBA after enrichment) + SN B †/ - BoNT B producing-C. botulinum
BoNT gene (qPCR) + C. botulinum type B
2/ Ham (big) 27-March BoNT (MBA with trypsin treatment) BoNT detected
C. botulinum (MBA after enrichment) + SN B †/ - BoNT B producing-C. botulinum
BoNT gene (qPCR) + C. botulinum type B
3/ Serum 4 30-March BoNT (MBA) + SN B specific mild symptoms 1, 2, 3 BoNT B detected
3/ Faeces 2 31-March BoNT (MBA) - BoNT Not detected
C. botulinum (MBA after enrichment) - BoNT Not detected
BoNT gene (qPCR) - BoNT Not detected
4/ Serum 5 6-Apr BoNT (MBA) + SN B specific mild symptoms 1, 2, 3 BoNT B detected
4/ Faeces 3 7-Apr BoNT (MBA) - BoNT Not detected
C. botulinum (MBA after enrichment) - BoNT Not detected
BoNT gene (qPCR) - BoNT Not detected
5/ Serum 6 21-Apr BoNT (MBA) - BoNT Not detected

1 BoNT detection by mouse bioassay (MBA), + SN ABE-F or + SN B: BoNT mouse bioassay with seroneutralisation using botulism antitoxins type anti ABE-F or anti B.
2 -: no symptom, † death or specific signs compatible for botulism 1 for visible abdominal hypotonicity, 2 for touch abdominal hypotonicity, 3 for the characteristic wasp size (retracting the abdomen) .

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