Fig. (4) Cellular association of aluminium adjuvant determined by flow cytometry. Human peripheral leucocytes were incubated with a low concentration (12.5 μg/ml) of lumogallion labelled Alhydrogel® or with non-labelled Alhydrogel® before the cells were stained with fluorescently labelled antibodies against T-lymphocytes, monocytes and granulocytes. Lymphocytes, monocytes and granulocytes were identified by FSC x SSC dot plots and after gate settings on respective populations, their association with fluorescent ABAs was determined. A-C; Cells incubated in the presence of non-labelled Alhydrogel®. D-F; Cells incubated in the presence of lumogallion labelled Alhydrogel®. A and D; T-lymphocytes identified by antibodies against CD3. B and E; Monocytes identified by antibodies against CD14. C and F; Granulocytes identified by antibodies against CD11b.