Fig. (6C) Mean fluorescence intensity from stained THP-1 cells in culture. Cytoplasm stained THP-1 cells continuously cultured after separation from lumogallion labelled adjuvants in the culture medium. At various days, samples were withdrawn, analysed by flow cytometry and the mean fluorescence intensity (MFI) was expressed as % of MFI obtained immediately after separation from free ABAs in the culture medium (Day 0). Experimental conditions as described in the Materials and Methods section. Left graph: Cells incubated with Alhydrogel^®; lumogallion fluorescence (■) and CellTrace™ Far Red fluorescence (❍). Right graph: Cells incubated with Adju-Phos^®; lumogallion fluorescence (■) and CellTrace™ Far Red fluorescence (❍).