Table 3: Cellular MTT activity after culture of cells in the presence of Al-adjuvants, zeolites or depleted growth medium.
THP-1 cells were cultured in the presence of ABAs, zeolites or in depleted culture medium. At day 1 respectively day 2 the cell numbers were counted and an MTT assay of the various incubations was performed. The MTT activity (absorbance) per 1cells was calculated and to normalize the samples, the ratio between MTT-activity in plain culture medium was assessed for each separate day. Data presented as mean +/- SD of three independent experiments.


Sample Day 1
Normalized MTT activity
Day 2
Normalized MTT activity
Alhydrogel®, 100 µg/ml 1.10 ± 0.12 1.42 ± 0.15
Adju-Phos®,100 µg/ml 1.18 ± 0.09 1.47 ± 0.12
NAY, 30 µg/ml 1.39 ± 0.17 1.42 ± 0.21
USY, 10 µg/ml 1.01 ± 0.09 1.10 ± 0.10
Depleted medium 1.15 ± 0.15 1.20 ± 0.12