Fig. (3) A. Western blotting of nuclear extracts from DLBCL and MM-derived cell lines. Probing with the anti-FOXP1 monoclonal antibody JC12 shows a lack of FOXP1 protein expression in MM as compared with DLBCL samples. Weak expression of three FOXP1 protein isoforms was detectable in the JJN3 cell line. TBP was used to indicate sample loading. B. Double immunoenzymatic labelling of MM-derived cell lines; FOXP1 staining in brown and CD138 in blue. The Presence of occasional FOXP1+CD138+ double labelled cells was detected in RPMI8226 cells, whereas no FOXP1 labelling was detectable in the NCIH929 cell line.