Fig. (1) Stimulation of TLR-7 leads to protection against
spontaneous apoptosis. CLL cells (2 x 106/ ml) from three
different patients were incubated for 48 h in the presence or not of
Resiquimod 1µM. At the end of the incubation period, aliquots
were collected and the amount of nucleosomes in the cytoplasm
was quantified by the “Cell Death detection kit “ (Roche) as
described in Materials and Methods. Results from triplicate samples
are expressed as % of enrichment in cytoplasmic nucleosomes, in
comparison with control unstimulated cells taken as 100%. The
differences between control and Resiquimod-treated cells were
analysed with the Student’s t test. * p ; 0.05 ;** p ; 0.01