Fig. (2) The TLR-7 ligand Resiquimod inhibits caspase 8 activity in CLL cells. CLL cells were incubated for 20 h in the presence of medium alone or with 1 µM or 10 µM of Resiquimod. Caspase 8 activity in cell lysates was estimated by cleavage of the fluorescent substrate IETD-AMC over an 80 minutes period as described in Materials and Methods. The specificity of the reaction was assessed by the abolition of the activity observed in the presence of the specific caspase 8 inhibitor IETD-CHO.