Fig. (2) The TLR-7 ligand Resiquimod inhibits caspase 8
activity in CLL cells. CLL cells were incubated for 20 h in the
presence of medium alone or with 1 µM or 10 µM of Resiquimod.
Caspase 8 activity in cell lysates was estimated by cleavage of the
fluorescent substrate IETD-AMC over an 80 minutes period as
described in Materials and Methods. The specificity of the reaction
was assessed by the abolition of the activity observed in the
presence of the specific caspase 8 inhibitor IETD-CHO.