Fig. (6) Resiquimod and Isiquimod triggers the expression of
BAFF and BAFF-R in CLL cells. A/ Leukemia cells from one
CLL patient were incubated for 20 h in the presence of medium
alone (C; control) or with varying concentrations of Resiquimod
(Rq) 1, 5 or 10 µM. At the end of the incubation time, cells were
lysed and the content in BAFF was analysed by Western blot as
described in Materials and Methods, using Δ-actin as internal
control of equal protein loading. B and C/ Same as previously, but
cells were incubated with Resiquimod (Rq) 1 µM or 10 µM,
Imiquimod (Iq) 1µM or 10 µM, or BAFF 250 ng/ml. Cell lysates
were analyzed as before for the expression of BAFF (B) and BAFFR
(C).