Fig. (6) Resiquimod and Isiquimod triggers the expression of BAFF and BAFF-R in CLL cells. A/ Leukemia cells from one CLL patient were incubated for 20 h in the presence of medium alone (C; control) or with varying concentrations of Resiquimod (Rq) 1, 5 or 10 µM. At the end of the incubation time, cells were lysed and the content in BAFF was analysed by Western blot as described in Materials and Methods, using Δ-actin as internal control of equal protein loading. B and C/ Same as previously, but cells were incubated with Resiquimod (Rq) 1 µM or 10 µM, Imiquimod (Iq) 1µM or 10 µM, or BAFF 250 ng/ml. Cell lysates were analyzed as before for the expression of BAFF (B) and BAFFR (C).