Fig. (7) Resiquimod stimulates sBAFF and sAPRIL release by
CLL cells. Leukemia cells were adjusted at 4 × 106/ml in complete
RPMI-1640 medium and allowed to incubate for 48 h in the
presence or not of Resiquimod 0.1 µM or 1 µM. The concentration
of soluble BAFF, sBAFF, released in the supernatant was
determined from triplicate samples with the sBAFF kit (R &D)
using purified sBAFF for the calibration. The concentration of
soluble APRIL, sAPRIL, was determined with the human APRIL
ELISA kit (AbCys). The Student’s t test was used to compare the
values between control and Resiquimod-treated cells: * p < 0.05 ;
**p < 0.01 ; *** p < 0.001. Results are from one representative
CLL patient out of three that gave comparable results.
