Fig. (8) Resiquimod stimulates NF-B activation in CLL cells.
Leukemia cells from two representative CLL patients were
suspended at 4 x 106/ml and cultured in the presence of medium
alone or with Resiquimod 1 µM or 10 µM. After 6 hours of
incubation, cells were collected, washed and nuclear extracts were
prepared. The activation of the different NF-κB members was
estimated by measuring their binding to a consensus NF-κB
sequence with the Transam NFκB family ELISA kit, as described
in Materials and Methods. Results are expressed as the percentage
of binding in comparison with control unstimulated cells taken as
100%.