Fig. (8) Resiquimod stimulates NF-B activation in CLL cells. Leukemia cells from two representative CLL patients were suspended at 4 x 106/ml and cultured in the presence of medium alone or with Resiquimod 1 µM or 10 µM. After 6 hours of incubation, cells were collected, washed and nuclear extracts were prepared. The activation of the different NF-κB members was estimated by measuring their binding to a consensus NF-κB sequence with the Transam NFκB family ELISA kit, as described in Materials and Methods. Results are expressed as the percentage of binding in comparison with control unstimulated cells taken as 100%.