Fig. (9) Resiquimod stimulates the activation of members of the AP-1 family. Leukemia cells from one representative CLL patient, out of three that gave comparable results, were treated as for NF-κB determination and the presence of various members of the AP-1 family of transcription factors in nuclear extracts was tested with the Transam AP-1 family ELISA kit (Active Motif) as described in Materials and Methods.