Fig. (3) Flow cytometric analyses of the established cell lines (GDLB-GCB1 and GDLB-GCB2). The original tumor samples and the established cell lines (GDLB-GCB1 and GDLB-GCB2) demonstrated the positive expression of CD10, CD19, CD20 and surface immunoglobulin and the negative expression of CD5 (a, b). Surface CD40L expression was the negative expression in GDLB-GCB 1 and weakly positive in GDLB-GCB 2 (c). CD40L+3T3 was used for a positive control of CD40L (c). Abbreviation: CD40L, CD40 ligand; CD40L+3T3, NIH Swiss mouse embryonic fibroblast cell line transfected with CD40L.