Table 1: In vitro IC50 values of test compounds 8a8d, 14 and 16 for TcAChE, eqBuChE and DPPH.



Compounds AChE
IC50 (nM)
BuChE
IC50 (nM)
(SI)a DPPH IC50 (µM)
8a (n = 2) 1530 128.82 11.87 46.23
8b (n = 3) 1530 12.58 121.62 11.48
8c (n = 4) 2200 11.22 196.07 14.38
8d (n = 6) 49.31 4.74 10.40 12.67
14 17.37 3.16 5.49 129.41
16 >100 µM 1698.24 >58.88 125.89
Tacrine 102 14.12 7.22 nd
Donepezil 220 2870 0.08 nd
Trolox nd nd - 17.57

aBuChE selectivity index = IC50(TcAChE)/IC50(eqBuChE). n.d. = not determined.