Fig. (6) Realease of AIF and activation of caspases after ABQ-48 and NBQ-48 24 hr treatments. (A) Western blot shows lysates of Toledo non-Hodgkin's B cell lymphoma cell line (ATCC CRL2631). A specific band was detected for AIF at approximately 57 kDa (as indicated). (B) At least three independent experiments were performed and data shown are the mean±SD (n=6). ***p < 0.0001 compared to the drugs treated group. (C) Western blot analysis of caspase 3 and 8, results showed presence of caspases 3. (D) Activity of caspase 3 was detected, significant changes were observed in Caspase-3 antibody endogenous levels of full length caspase-3 (35 kDa) and the large fragments of caspase-3 resulting from cleavage (17 and 19 kDa).