| Primers |
Sequence (5´-3´) |
Tm | %GC | PCR product (bp) |
Target sequence. Access Number GenBank |
|---|---|---|---|---|---|
| 5´ Alu-p53 | GGG TAA GGG CGG TTG TCA GT | 63.5 | 60 | 74 | U38676 |
| 3´Alu-p53 | GGG AGG TCA AAT AAG CAG AAG GAG | 63.6 | 50 | ||
| 5´ Alu-Glob | CCT GGC TGG ATG TTG TGG | 61.1 | 61.1 | 50 | AF057392 |
| 3´ Alu-Glob | GCC TCC TAA AGT GCT GGG TT | 61.8 | 52.3 | ||
| 5´ B-actin | TTA GTT GCG TTA CAC CCT TTC TTG | 61.7 | 41.6 | 152 | M10277 |
| 3´ B-actin | TCA CCT TCA CCG TTC CAG TTT | 61.8 | 47.6 |