Table 2: RT-PCR standardization for Alu-p53. Effect of the concentrations of MgCl2 and primers on Ct (threshold cycle, defined as the number of cycles required for the fluorescent signal to cross the threshold).
Template |
[MgCl2] |
[primers] |
Ct |
pDNA 1 x107 copies/µl |
1.5mM |
150 nM |
11,63 |
100 nM |
12,75 |
75 nM |
14,99 |
NTC |
150 nM |
35,42 |
100 nM |
36,36 |
75 nM |
37,07 |
pDNA 1 x107 copies/µl |
1.25mM |
150 nM |
13,4 |
100 nM |
18,53 |
75 nM |
19,06 |
NTC |
150 nM |
37,52 |
100 nM |
40 |
75 nM |
40 |
pDNA 1 x107copies/µl |
1mM |
150 nM |
30,54 |
100 nM |
40 |
75 nM |
40 |
NTC |
150 nM |
40 |
100 nM |
40 |
75 nM |
40 |
pDNA 1 x107 copies/µl |
0.75mM |
150 nM |
40 |
100 nM |
40 |
75 nM |
40 |
NTC |
150 nM |
40 |
100 nM |
40 |
75 nM |
40 |
pDNA 1 x107copies/µl |
0.5mM |
150 nM |
40 |
100 nM |
40 |
75 nM |
40 |
NTC |
150 nM |
40 |
100 nM |
40 |
75 nM |
40 |