Standardization of DNA Residual Quantification Method of Vero Cell Rabies Vaccine for Human Use

Table 3: RT-PCR standardization for Alu-Glob. Effect of the concentration of MgCl2 and primers on Ct.

Template	*[MgCl₂] and [primers]*	Ct
pDNA 1 x10⁷ Copies/µl	1.75mM - 50 nM	17,87
gDNA Vero 50ng/µl		27,36
NTC		36,68
pDNA 1 x10⁷ Copies/µl	1.5mM - 50 nM	21,29
gDNA Vero 50ng/µl		30,98
NTC		40
pDNA 1 x10⁷ Copies/µl	1.25mM – 75 nM	22,65
gDNA Vero 50ng/µl		32,33
NTC		40
pDNA 1 x10⁷ Copies/µl	1.25mM – 100 nM	19,92
gDNA Vero 50ng/µl		25,01
NTC		40