Table 7:
Effect of VAHV I volume template on amplification (Ct).
Sample
Volume of template added (ul)
Ct
pDNA 1x10
7
2
21.24
gDNA 100ng/µl
2
35.41
VAHV I 4x10
5
copies/µl
2
35.01
4
40
6
40
8
40
10
40
VAHV I W/OUT DNA
2
40
4
40
6
40
8
40
10
40
NTC
0
40