Table 4: Some characteristics of enzymes used for ADEPT systems.

Enzyme Source Reaction Specificity
AP Calf intestine Hydrolysis of phosphate groups from aliphatic and aromatic substrates
CPA Bovine pancreas Cleavage of α-glutamyl-peptides, e.g. Methotrexate release from methotrexate-α-peptides.
CPG2 Pseudomonas sps. Cleavage of amidic, oxycarbonyl and carbamic bonds located between an l-glutamyl moiety and an aromatic nucleus.
CD Baker’s yeast Catalyses the deamination of cytosine to uracil
α-g Mammalian Hydrolysis of α-galactosyl-linked residues; used with a self-immolative linker
β-g E. coli Hydrolysis of β-galactosyl-linked residues
β-G E. coli (type X-A); E. Coli K12 Hydrolysis of glucuronides linked to various substrates
β-glu Sweet almonds Hydrolysis of β-glucose-linked residues
β-L Enterobactercloace strain 256A;
B. cereus
Cleavage of the 4-membered lactam of cephalosporin: effects elimination of substituents appended to 3′-position of cephalosporin substrates
NR E. coli Reduction of nitro groups in some aromatic systems
PGA E. coli Cleavage of the phenyloxyacetamide groups linked
PVA Fusariumoxysporum Cleavage of the phenyloxyacetamide groups linked to various substrates