Table 1: Time scedule for elution.

Time (min) Flow rate (mL/min)
0.00-7.20 1.0
7.20-8.20 1.0-1.5
8.21-11.00 1.5
11.00-12.00 1.5-2.0
12.01-20.00 2.0
20.00-21.00 2.0-1.0

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