Table 2: System suitability data (n=6) of a standard solution containing salicylic acid, 2,3-dihydroxybenzoic acid, 2,4-dihydroxybenzoic acid, 2,5-dihydroxybenzoic acid and 3,4-dihydroxybenzoic acid (5 μg/mL for each component), measured at 247 nm.

Standards tR (min) RSD% for tR Area RSD% for area k Symm
3,4-DHBA 3.861 2.47 343.53 7.44 1.970 0.80
2,5-DHBA 6.320 2.49 152.32 9.30 3.862 0.77
2,3-DHBA 8.876 0.62 120.57 4.33 5.828 0.75
2,4-DHBA 9.788 1.49 277.28 7.42 6.529 0.83
SA 17.965 1.11 137.28 8.49 12.819 0.83

tR: retention time of compound
RSD%: relative standard deviation (%)
k: retention factor
Symm: symmetry factor
DHBA: dihydroxybenzoic acid; SA: salicyclic acid

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