| Validation Parameter | Result |
|---|---|
| LOQ | 0.023 µmol/L |
| LOD | 0.009 µmol/L |
| Calibration (y = mx + b) | y=2.419-0.0355 |
| R2 | 0.9982 |
| Accuracy of back-calculated calibrator points (n=32) | 88.4-108.0% |
| Accuracy (intra-day) | - |
| level 1 (0.347 µmol/L) | 93.6% |
| level 2 (0.591 µmol/L) | 99.4% |
| level 3 (20.6 µmol/L) | 103% |
| level 4 (44.1 µmol/L) | 106% |
| Reproducibility (intra-day) | - |
| level 1 (0.347 µmol/L) | 10.0% |
| level 2 (0.591 µmol/L) | 5.4% |
| level 3 (20.6 µmol/L) | 11.3% |
| level 4 (44.1 µmol/L) | 3.7% |
| Accuracy (inter-day) | - |
| level 1 (0.347 µmol/L) | 99.0% |
| level 2 (0.591 µmol/L) | 106% |
| level 3 (20.6 µmol/L) | 88.1% |
| level 4 (44.1 µmol/L) | 104% |
| Reproducibility (inter-day) | - |
| level 1 (0.347 µmol/L) | 10.4% |
| level 2 (0.591 µmol/L) | 14.4% |
| level 3 (20.6 µmol/L) | 3.9% |
| level 4 (44.1 µmol/L) | 5.2% |
| Freeze-thaw stability (3 cycles) | - |
| level 2(0.591 µmol/L) | 102-115% |
| level 4 (44.1 µmol/L) | 95.7-107% |