Fig. (5) Concentration dependent inhibition of DNA synthesis by NSC 176327 and NSC 305831in a polymerase stop reaction using
the human telomeric sequence [TTAGGG]4.A. Lane 1, Temp [TTAGGG]4 with 1 µM BRACO19;lane 2, labeled primer at 15 nM, lane 3;
Temp [TTAGGG]4,lane 4; Temp [TTAGAG]4;and lane 5, Temp [TTAGAG]4 with NSC 176327 at 5 µM. The concentrations of NSC 176327
were 0.1, 0.25, 0.5, 2.5, 5, 10, 25, and 50 μM. B. Lane 1, Temp [TTAGGG]4 with 1 µM BRACO19;lane 2, labeled primer at 15 nM, lane 3;
Temp [TTAGGG]4,lane 4; Temp [TTAGAG]4;and lane 5, Temp [TTAGAG]4 with NSC305831 at 5 µM.The concentrations of NSC 305831
were 10, 15, 20, 22.5, 25, 27.5, 30, 35, and 40 μM.
