Nitropropenyl Benzodioxole, An Anti-Infective Agent with Action as a Protein Tyrosine Phosphatase Inhibitor

Table 1: Change in permeability of E. coli exposed to NPBD for 5 min as measured by uptake of fluorescent NPN into the outer membrane lipid bilayers.

Treatment	Average RFU^{^}	NPN-uptake value^*
NPBD	2091 ±665	0.61 ±0.19
PolymyxinB	10018 ±627	2.92 ±0.18
EDTA	4703 ±974	1.37 ±0.28
Untreated Cells	3645 ±384	1.06 ±0.11
HEPES	3429 ±74	1.00 ±0

^{^} Average RFU were calculated by subtracting fluorescence of identical wells without NPN,

^* NPN-uptake value is a ratio of relative fluorescence of the test well containing NPBD, Polymyxin B or EDTA, and the relative fluorescence of the control well containing HEPES buffer alone. EDTA and polymyxin B are positive controls which disrupt the OM allowing penetration of NPN into the lipid bilayer.