Fig. (2) Drug susceptibility assays against non-albicans species of Candida in the presence of MB. (a) Spot assay of C. krusei and C. tropicalis; in the absence (control) and presence of MB. (b) Disc diffusion assay against C. krusei and C. tropicalis; and their respective zone of inhibitions in the absence (control) and presence of MB. For control, discs were spotted with the solvent of MB (water). (c) Broth microdilution assay to determine the MIC₈₀ of C. krusei and C. tropicalis in presence of MB. Data is quantitatively displayed with colour (see colour bar), where each shade of colour represents relative optical densities of the cell. The minimum drug concentration that inhibits growth by 80% relative to the drug-free growth control is indicated as MIC₈₀ for each strain.