Table 1: Primers used to amplify the cDNA of the IDS without the sequence it encodes for the native signal peptide. IDS-F (28 nucleotides) contains an EcoRI recognition site (underlined). IDS-R (26 nucleotides), contains a Not I recognition site (underlined).

Primer Sequence 5´- 3´ Number of nucleotides

T7

  TAATACGACTCACTATAGGG

20

SP6

  TATTTAGGTGACACTATAG

19

IDS-F

  GGAATTCTCCGAAACGCAGGCCAACTCG

28

IDS-R



  GGCGGCCGCACACTGAGGGATGTCTG



26