Evaluation in Mouse Model of Combined Virus-bacterial Vaccine Based on Attenuated Influenza A(H7N3) Virus and the Group B Streptococcus Recombinant Polypeptides

Table 1: The LAIV reproduction in mice after primary vaccination with 10⁶ EID₅₀ infectious doses*.

Vaccine groups	Day 3		Day 6
Vaccine groups	Mean virus titers (log₁₀ EID₅₀/ml) ± SEM	Number infected/total vaccinated	Mean virus titers (log₁₀ EID₅₀/ml) ± SEM	Number infected/total vaccinated
Virus isolation from lungs
LAIV	2.3±1.1	1/4	1.5±0.0	0/4
LAIV+GBSV	2.4±1.3	1/4	1.5±0.0	0/4
Virus isolation from nasal turbinates
LAIV	2.6±1.1	3/4	1.8±0.3	1/4
LAIV+GBSV	2.7±1.2	3/4	1.5±0.0	0/4

* - Lungs and nasal tissues were collected 3 or 6 days post infection (p.i.) and titrated in eggs for assessing viral replication. The virus titers are expressed as the mean log₁₀ EID₅₀/ml±SEM from four mice per group. The limit of virus detection was 10^1.5 EID₅₀/ml. Tissues in which no virus was detected were given a value of 10^1.5 EID₅₀/ml for calculation of the mean titer. Mice were considered infected if infectious virus was detected in 0.1 ml of 1:10 dilution of tissue homogenate.