Table 1: Composition of reaction mixture for PCR used for amplification of mecA gene.

Reagents	Vol (μl)
10x buffer	2.5
dNTP mix (25mM each)	0.25
MgCl2 (25mM)	1
Primer mix (130mg/ml each)	0.25
Taq polymerase (1 U/μl)	0.25
PCR water	15.75
Template DNA	5
Total volume	25