| Article | Studying | Results | P value |
|---|---|---|---|
| Schlievert, et al., 1998 [13] | Asc10 and EBS | The combination of Asc10 and EBS increase lethality. | 0.0002 |
| Chuang, et al., 2008 [4] | Asc10 | A double RGD mutant exhibited virulence equivalent to that of a strain carrying the prgB null allele. | ≤ 0.01 |
| The N-terminal RGD motif is more important for virulence than the C-terminal RGD motif. | ≤ 0.01 | ||
| Strains with mutations to the aggregation domains are more virulent than double RGD motif mutants. | ≤ 0.01 | ||
| Chuang-Smith, et al., 2010 [5] | Asc10 | After 4 hours the Asc10- strain colonized heart valves at 40% the level of the Asc10+ strain. | n/a* |
| Several mutations had less severe impact on virulence in the ex vivo model. | ≤ 0.02 | ||
| Chow, et al., 1993 [14] | Asa1 and hemolysin | Strains expressing Asa1 and hemolysin exhibited the highest rabbit mortality. | < 0.01 |
| Asa1+ strains produces vegetations weighing more than those produced by Asa1- strains. | < 0.01 | ||
| Haller, et al., 2014 [15] | Cell-wall glycolipids | bgsA mutants produced vegetations that weighed less than the wild type. | < 0.35 |
| bgsB mutants produced vegetations that weighed less than the wild type. | < 0.05 | ||
| bgsA and bgsB mutants produced vegetations that contained less CFU/gram than the wild type. | < 0.0159 | ||
| Nannini, et al., 2005 [7] | Gls24 | A Gls24 mutant was outnumbered by its parental strain at both time points in an endocarditis model. | < 0.001 |
| Nallapareddy, et al., 2006 [9] | Ebp pili | Mutant strains exhibited markedly reduced biofilm formation. | < 0.001 |
| Mutant strains were attenuated compared to the parental strain in a 2 hours in vitro attachment assay. | < 0.001 | ||
| An EbpA mutant was outnumbered by its parental strain in an endocarditis model. | 0.0069 | ||
| Thurlow, et al., 2010 [6] | GelE | Mutations to GelE significantly decreased the mean CFU per gram of heart tissue | < 0.05 |
| Mutations to GelE significantly increases the matrix layer | < 0.05 | ||
| Singh, et al., 2010 [3] | Ace | Ace- strains were highly attenuated in adherence to collagen type I and IV compared to Ace+ strains | < 0.0001 |
| OG1RFΔace was outnumbered by OG1RF in a mixed endocarditis model | < 0.0001 | ||
| At 4 hours pAT392 produced vegetations with less CFU/geometric mean than pAT392::ace | 0.0417 | ||
| Singh, et al., 2015 [2] | EfbA | TX5707 mutant showed a 44% decrease in whole cell binding to immobilized fibronectin | < 0.0001 |
| TX5707 produced biofilms with a 15% reduced density compared to OG1RF and TX5708 | < 0.0001 | ||
| At 48 hours TX5707 was outnumbered by OG1RF in a mixed endocarditis model | < 0.0006 | ||
| At 48 hours TX5707 was outnumbered by TX5708 om a mixed endocarditis model | < 0.0086 | ||
| Frank, et al., 2011 [16] | Eep | OG1RFΔeep exhibited a 4-log10 decrease in valve bacterial load compared to OG1RF | 0.003 |
| OG1RFΔeep produced smaller vegetations (mean 8.8 mg) than OG1RF (mean 47.6 mg) | 0.0065 | ||
| A complemented strain produced larger vegetations (mean 32.1) than OG1RFΔeep (mean 8.8 mg) | 0.027 | ||
| * n/a indicates that the corresponding p-value is not presented in the article. | |||