Table 2: Catalase activity of S. marcescens cell lysates following centrifugation to separate cell debris (pellet) from soluble components (supernatant). Activity was determined in stationary phase cells (48h cultures, N = 6) or exponential phase cells (6h cultures, N = 7) as units/ml as well as normalized to sample protein concentrations (U/mg). Protein concentrations were not determined for pelleted debris due to catalase activities indistinguishable from background.
|
Stationary |
Exponential |
| Strain |
Supernatant (U/ml) |
Pellet (U/ml) |
Supernatant (U/mg) |
Supernatant (U/mg) |
| SM-R |
103.96±3.21 |
2.28±0.47 |
779.37±204.17 |
ND |
| SM-W |
123.89±21.39 |
4.46±1.04 |
852.28±111.46 |
ND |
| SM-01 |
149.49±25.49 |
4.50±0.82 |
522.26±31.64 |
749.79±67.24 a,b
|
| SM-02 |
135.32±17.35 |
3.81±0.79 |
654.1±3.94 |
904.63±50.28 a,b
|
| SM-06 |
84.17±11.76 |
2.06±0.14 |
541.43±49.98 |
625.59±19.66 |
a: significantly different from SM during stationary phase; b: significantly different from SM-06; ND: not determined