Gene type |
Primer |
Nucleotide sequence |
Annealing Temp |
Amplicon size (bp) |
Carabapenemase genes internal primers |
OXA-48 |
F |
5`– AAGTGTGCAACGCAAATGGC – 3` |
55°C |
137 |
R |
5` – CTGTTCCAGATCTCCATTCC – 3` |
IMP |
F |
5` – GTTAGTCACTTGGTTTGTG – 3` |
50°C |
103 |
R |
5` – CGAGAATTAAGCCACTCTA – 3` |
KPC |
F |
5` – ATTCGCTAAACTCGAACAG – 3` |
50°C |
130 |
R |
5` – AAGAAAGCCCTTGAATGAG – 3` |
VIM-1 |
F |
5` – GAGCTCTTCTATCCTGGTG – 3` |
52°C |
103 |
R |
5` – CTTGACAACTCATGAACGG – 3` |
NDM-1 |
F |
5` – ACTTCCTATCTCGACATGC – 3` |
52°C |
133 |
R |
5` – TGATCCAGTTGAGGATCTG – 3` |
Carabapenemase genes full length primers |
OXA-48 |
F |
5` – TTGGTGGCATCGATTATCGG – 3` |
55°C |
743 |
R |
5` –GAGCACTTCTTTTGTGATGGC – 3` |
VIM-1 |
F |
5` – TGTTATGGAGCAGCAACGATG – 3` |
56 °C |
920 |
R |
5` – AAAGTCCCGCTCCAACGATT – 3` |
NDM-1 |
F |
5` – TGTTATGGAGCAGCAACGATG – 3` |
62°C |
795 |
R |
5` – AAAGTCCCGCTCCAACGATT – 3` |
Housekeeping gene |
RpoD |
F |
5` – CGAACTGCTTGCCGACTT – 3` |
56°C |
131 |
R |
5` – GCGAGAGCCTCAAGGATAC – 3` |