Table 5: Parameters of accuracy of serological tests for diagnosis of paracoccidioidomycosis. Detection of serum antigens. Cross reactions with other diseases.
| Accuracy parameters |
| Method |
Monoclonal antibodies |
S |
E* |
PPV |
NPV |
A |
PLR |
NLR |
Ref. |
| Inhi-ELISAa |
FL |
80.0 |
100.0 |
100.0 |
69.0 |
86.0 |
20.0 |
0.2 |
357 |
| Inhi-ELISAb |
gp43 |
96.2 |
96.7 |
96.2 |
96.7 |
96.5 |
32.0 |
0.03 |
358 |
| Inhi-ELISAc |
gp43 / gp70 |
90.9 |
100.0 |
100.0 |
96.7 |
97.5 |
30.0 |
0.09 |
360 |
| Cross reactions** |
| Method |
Monoclonal antibodies |
TBC |
HST |
ASP |
CRC |
LSZ |
CCD |
ESP |
Ref. |
| Inhi-ELISAa |
FL |
55.5 |
40.0 |
100.0 |
10.0 |
… |
… |
11.1 |
357 |
| Inhi-ELISAb |
gp43 |
… |
0.0 |
… |
0.0 |
… |
… |
… |
358 |
| Inhi-ELISAc |
gp43 / gp70 |
… |
… |
… |
… |
… |
… |
… |
360 |
*Evaluated in healthy donors; ** Evaluated in patients with microbiologically confirmed active disease; Inhi-ELISA: Inhibition-ELISA; cut-off - a: >3.3µg/mL; b:>1.353µg/mL; c: 0.23g/mL for gp43 and 0.97g/mL for gp70; FL: culture filtrate antigen yeast phase; S: sensitivity, E: specificity; PPV / NPV: positive and negative predictive values; A: accuracy; PLR: positive likelihood ratio; NLR: negative likelihood ratio; TBC: tuberculosis; HST: histoplasmosis; ASP: aspegillosis; CRC: cryptococcosis; LSZ: lacaziosis; CCD: coccidioidomycosis; ESP: esporotrichosis.