| MRSA identification | ||||
|---|---|---|---|---|
| Primer | Oligonucleotide Sequence (5'- 3') | Target gene/position in genome | Amplicon Size (bp) | Reference |
| Staph 756F Staph 756R |
-AACTCTGTTATTAGGGAAGAACA- -CCACCTTCCTCCGGTTTGTCACC- |
16S rRNA | 756 | McClure et al., 2006 |
| MecA1-F MecA2-R |
-GTAGAAATGACTGAACGTCCGATAA- -CCAATTCCACATTGTTTCGGTCTAA- |
mecA | 310 | |
| Luk-PV-1F Luk-PV-2R |
-ATCATTAGGTAAAATGTCTGGACATGATCCA- - GCATCAAGTGTATTGGATAGCAAAAGC- |
lukS/F-PV | 433 | |
| SCCmec typing | ||||
| Type I-F Type I-R |
-GCTTTAAAGAGTGTCGTTACAGG- -GTTCTCTCATAGTATGACGTCC- |
SCCmec I | 613 | Zhang et al., 2005 |
| Type II-F Type II-R |
-CGTTGAAGATGATGAAGCG- -CGAAATCAATGGTTAATGGACC- |
SCCmec II | 398 | |
| Type III-F Type III-R |
-CCATATTGTGTACGATGCG- -CCTTAGTTGTCGTAACAGATCG- |
SCCmec III | 280 | |
| Type IVa-F Type IVa-R |
-GCCTTATTCGAAGAAACCG- -CTACTCTTCTGAAAAGCGTCG- |
SCCmecIVa | 776 | |
| Type IVb-F Type IVb-R |
-TCTGGAATTACTTCAGCTGC- -AAACAATATTGCTCTCCCTC- |
SCCmecIVb | 493 | |
| Type IVc-F Type IVc-R |
-ACAATATTTGTATTATCGGAGAGC- -TTGGTATGAGGTATTGCTGG- |
SCCmecIVc | 200 | |
| Type IVd-F Type IVd-R |
-CTCAAAATACGGACCCCAATACA- -TGCTCCAGTAATTGCTAAAG- |
SCCmecIVd | 881 | |
| Type V-F TypeV-R |
-GAACATTGTTACTTAAATGAGCG- -TGAAAGTTGTACCCTTGACACC- |
SCCmec V | 325 | |
| MecA147-F MecA147-R |
-GTGAAGATATACCAAGTGATT- -ATGCGCTATAGATTGAAAGGAT- |
mecA | 147 | |
| Spa typing | ||||
| SPA 1 | -GATTTTAGTATTGCAATACATAATTCG- | 114-140 | Schmitz et al., 1998 | |
| SPA 2 | -CCACCAAATACAGTTGTACCG- | 1702-1682 | ||
| SPA 3 | -CTTTGGATGAAGCCGTTGCGTTG- | 1088-1066 | ||
| Spa sequencing | ||||
| 1095F | -AGACGATCCTTCGGTGAGC- | NA | NA | Larsen et al., 2008 |
| 1517R | -GCTTTTGCAATGTCATTTACTG- | NA | NA | |
| Agr typing | ||||
| Pan-agrB (F) | -ATGCACATGGTGCACATGC- | agrB | - | Shopsin et al., 2003 |
| agr I (R) | -GTCACAAGTACTATAAGCTGCGAT- | agrD | 440 | |
| agr II (R) | -GTATTACTAATTGAAAAGTGCCATAGC- | agrC | 572 | |
| agr III (R) | -CTGTTGAAAAAGTCAACTAAAAGCTC- | agrD | 406 | |
| agr IV (R) | -CGATAATGCCGTAATACCCG- | agrC | 588 | |
| Multilocus sequence typing | ||||
|
arcC-fd arcC-Rv |
-TTGATTCACCAGCGCGTATTGTC- -AGGTATCTGCTTCAATCAGCG- |
Carbamate kinase (arcC) | 500 | Enright et al., 2000 |
|
aroE-fd aroE-Rv |
-ATCGGAAATCCTATTTCACATTC- -GGTGTTGTATTAATAACGATATC- |
Shikimate dehydrogenase (aroE) |
500 | |
|
glpF-fd glpF-Rv |
-TGGTAAAATCGCATGTCCAATTC- -CTAGGAACTGCAATCTTAATCC- |
Glycerol kinase (glpF) | 500 | |
|
gmk-fd gmk-Rv |
-ATCGTTTTATCGGGACCATC- -TCATTAACTACAACGTAATCGTA- |
Guanylate kinase (gmk) | 500 | |
|
pta-fd pta-Rv |
-GTTAAAATCGTATTACCTGAAGG- -GACCCTTTTGTTGAAAAGCTTAA- |
Phosphate acetyltransferase (pta) |
500 | |
|
tpi-fd tpi-Rv |
-TCGTTCATTCTGAACGTCGTGAA- -TTTGCACCTTCTAACAATTGTAC- |
Triosephosphate isomerase (tpi) |
500 | |
|
yqiL-fd yqiL-Rv |
-CAGCATACAGGACACCTATTGGC- -CGTTGAGGAATCGATACTGGAAC- |
Acetyl coenzyme A acetyltransferase (yqiL) | 500 | |