Table 3: Preliminary screening for antagonistic activity of Dead Sea mud isolates against E. coli 8739 and B. subtilis.
– |
Diameter of Inhibition zones (mm*) |
Isolate nr. |
Escherichia Coli 8739 |
Bacillus Subtilis 6633 |
1-DSM |
8.33 ± 0.67 |
3.67 ± 2.66 |
5-DSM |
7.66 ± 0.37 |
10.33 ± 1.45 |
7-DSM |
7.66 ± 0.67 |
9.67 ± 0.33 |
10-DSM |
0.00 ± 0.00 |
6.00 ± 1.15 |
12-DSM |
4.66 ± 0.00 |
0.00 ± 0.00 |
14-DSM |
4.67 ± 0.00 |
0.00 ± 0.00 |
18-DSM |
5.00 ± 0.00 |
0.00 ± 0.00 |
20-DSM |
0.00 ± 0.00 |
6.33 ± 1.45 |
21-DSM |
4.67 ± 0.00 |
0.00 ± 0.00 |
24-DSM |
9.00 ± 0.00 |
12.33 ± 0.45 |
Cm |
10.00 ± 0.60 |
26.00 ± 0.40 |
0.9% NaCl |
0.00 ± 0.00 |
0.00 ± 0.00 |
*Average of three replicates ± standard error of means, 10µl of OD600 ~1.0 isolates suspension spotted on plate previously seeded with bacterial strains; 10µl of 25mg/ml chloramphenicol (Cm) and 10µl of 0.9% NaCl were used as positive and negative control respectively.