Table 4: Some physiological properties of Dad Sea mud bacterial isolates.

Characteristics B. subtilis 6633 1-DSM 5-DSM 7-DSM 24-DSM
Gram staining + + + + +
Catalase + + + + +
Oxidase + + + + +
API 20E tests
Ortho NitroPhenyl-ßDGalactopyranosidase - - - - -
Arginine dihydrolase - - - - -
Lysine decarboxylase - - - - -
Ornithine decarboxylase + + + + +
Citrate utilization - - - - -
H2S production - - - - -
Urease + + + + +
Tryptophane deaminase - - - - -
Indole production + + + + +
Acetoin production + + + + +
Gelatinase + + + + +
NO2 production + + + + +
API 50CHB tests
Glycerol + + + + +
Erythritol - - - - -
D-Arabinose - - - - -
L-Arabinose + + + + +
Ribose + + + + +
D-Xylose + + + + +
L-Xylose - - - - -
Adonitol - - - - -
β-Methylxyloside - - - - -
Galactose - - - - -
D-Glucose + + + + +
D-Fructose + + + + +
D-Mannose + + + + +
L-Sorbose - - - - -
Rhamnose - - - - -
Dulcitol - - - - -
Inositol + + + + +
Mannitol + + + + +
Sorbitol + + + + +
α-Methyl-Dmannoside - - - - -
α-Methyl-D-glucoside + + + + +
N-Acetylglucosamine - - - - -
Amygdalin + + + + +
Arbutin + + + + +
Aesculin + + + + +
Salicin + + + + +
Cellobiose + + + + +
Maltose + + + + +
Lactose - - - - -
Melibiose - - - - -
Sucrose + + + + +
Trehalose + + + + +
Inulin - - - - -
Melezitose - - - - -
D-Raffinose - - - - -
Starch + + + + +
Glycogen + + + + +
Xylitol - - - - -
β-Gentiobiose + + + + +
D-Turanose + + + + +
D-Lyxose - - - - -
D-Tagatose - - - - -
D-Fucose - - - - -
L-Fucose - - - - -
D-Arabitol - - - - -
L-Arabitol - - - - -
Gluconate - - - - -
2-Ketogluconate - - - - -
5-Ketogluconate - - - - -

+: indicates positive reaction; - : indicates negative reaction.