Fig. (5) Effect of overexpression of Gp07, its domains and ΔGp07 on the morphology of host cell. (A) Morphology of wild type host cells (E. coli) under microscope at 100X magnification. (C) Morphology of E. coli cells overexpressing the control protein. (E,I,M,Q) Morphology of E. coli cells overexpressing Gp07, rNTD, rCTD and ΔGp07 respectively at 5h, as observed by phase contrast microscopy. (G,K,O,S) Morphology of E. coli cells overexpressing Gp07, rNTD, rCTD and ΔGp07 respectively at 8h, as observed by phase contrast microscopy. (B) DAPI stained normal host cells (E. coli BL21 DE3 not carrying any recombinant DNA) under fluorescence microscope. (D) DAPI stained E.coli cells overexpressing the control protein. (F,J,N,R) DAPI stained E.coli cells overexpressing Gp07, rNTD, rCTD and ΔGp07 respectively at 5h, under fluorescence microscope. (H,L,P,T) DAPI stained E. coli cells overexpressing Gp07, rNTD, rCTD and ΔGp07 respectively at 8h, under fluorescence microscope. All the scale bars are 5µm.

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