Table 1: Primer used in this study to express the Gp07, rNTD, rCTD and ΔGp07.

Name Sequence(5′-3′)# Purpose
C-gp07-F AAACCATGGGAATGTGGGTGTTGAGGAAAAAGGAGG Forward primer for synthesis of Gp07 and rNTD
C-gp07-R AAACTCGAGCGCTCCCCCTAAATTAGCTTCATAAC Reverse primer for Synthesis of Gp07 and ΔGp07
C-Bro-R AAACTCGAGGTCTGGATCTTTTAATGTTTGTTCAATTACATTG Reverse primer for the synthesis of rNTD
N-KilAC-F AAACATATGTACATCATTACAGTGTTGACTGAGTATAAGAAAG Forward primer for the synthesis of rCTD
N-gp07-R AAACTCGAGTTACGCTCCCCCTAAATTAGCTTCATAACC Reverse primer for the synthesis of rCTD
C-Δgp07-F AAACCATGGAAATGCAAGCATTACAAACATTTAATTTTAAAGAGC Forward primer for synthesis of ΔGp07
#Used primer, restriction sites are underlined; for PCR, Tm is calculated for the bases in bold.

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