Table 5: Clades obtained by MALDI-TOF MS with at least one actinobacterial isolate with antimicrobial activity. 16S rRNA molecular identification was obtained using SILVA Living Tree Project database. Sampling site and physicochemical pretreatment are included – U: Sonication; C: Calcium carbonate; M: Microwave; F: Phenol; T: Thermal; S: Direct Seeding.

Clade No. Isolates No. Bioactive Isolates Bioactive Isolate Pretreatment Sample Point Identification (98,7%)
9 5 4 439 U P1 Streptomyces hiroshimensis
559 C P4 Streptomyces lacticiproducens
736 C P4 Streptomyces hiroshimensis
775 M P4 Streptomyces hiroshimensis
20 31 1 636 M P2 Nonomuraea candida
22 1 1 388 M P2 Streptomyces werraensis
42 1 1 694 U P4 Streptomyces sp.
43 2 2 276 S P4 Streptomyces griseofuscus
290 C P1 Streptomyces albospinus
50 1 1 444 U P1 Streptomyces lunalinnaresii
54 3 2 652 M P4 Streptomyces sp.
515 S P4 Streptomyces sioyaensis
55 5 4 376 M P1 Streptomyces luteireticuli
445 U P1 Streptomyces sp.
571 F P1 Streptomyces luteireticuli
572 F P1 Streptomyces luteireticuli
57 6 6 288 C P1 Streptomyces sparsogenes
377 M P1 Streptomyces cinnamoneus
481 S P1 Streptomyces griseoaurantiacus
575 F P1 Streptomyces cinnamoneus
809 F P1 Streptomyces cinnamoneus
840 F P1 Streptomyces cinnamoneus
58 5 1 104 C P4 Streptomyces albolongus
59 19 9 247 S P1 Streptomyces sp.
397 M P2 Streptomyces morookaense
568 F P1 Streptomyces morookaense
612 M P1 Streptomyces luteosporeus
689 U P4 Streptomyces pseudoechinosporeus
745 F P1 Streptomyces morookaense
766 M P1 Streptomyces orinoci
837 C P1 Streptomyces lacticiproducens
864 T P4 Streptomyces lacticiproducens
60 29 21 5 S P1 Streptomyces lunalinnaresii
135 F P3 Streptomyces aureocirculatus
138 F P3 Streptomyces puniceus
160 M P1 Streptomyces mobaraensis
197 M P4 Streptomyces misionensis
201 U P1 Streptomyces lunalinnaresii
208 U P1 Streptomyces kasugaensis
326 F P1 Streptomyces mashuensis
381 M P1 Streptomyces ramulosus
448 U P2 Streptomyces albulus
479 S P1 Streptomyces mashuensis
516 S P4 Streptomyces sp.
531 C P1 Streptomyces hiroshimensis
616 M P1 Streptomyces yunnanensis
619 M P1 Streptomyces yunnanensis
626 M P1 Streptomyces lunalinnaresii
627 M P1 Streptomyces lydicus
659 U P1 Streptomyces yunnanensis
660 U P1 Streptomyces mobaraensis
723 C P1 Streptomyces albospinus
847 U P2 Streptomyces albospinus
61 1 1 213 U P1 Streptomyces lanatus
68 4 1 530 S P4 Streptomyces noursei
75 11 1 243 U P4 Streptomyces sparsogenes
78 4 3 365 F P4 Streptomyces lydicus
744 F P1 Streptomyces hygroscopicus
790 U P3 Streptomyces iranensis
79 4 1 805 C P3 Streptomyces sioyaensis
89 86 2 330 F P1 Streptomyces puniciscabei
356 F P4 Streptomyces corchorusii
90 345 13 67 C P2 Streptomyces puniciscabei
93 C P4 Streptomyces griseofuscus
112 F P1 Streptomyces lacticiproducens
126 F P2 Streptomyces padanus
152 F P4 Streptomyces albospinus
220 U P2 Streptomyces phaeoluteigriseus
292 C P1 Streptomyces murinus
297 C P2 Streptomyces lanatus
335 F P1 Streptomyces kebangsaanensis
382 M P1 Streptomyces puniciscabei
443 U P1 Streptomyces griseofuscus
663 U P1 Streptomyces cinnamoneus
691 U P4 Streptomyces sp.
91 48 3 140 F P3 Streptomyces misionensis
145 F P3 Streptomyces parvulus
246 S P1 Streptomyces graminisoli