Table 1: Scheme for preparing dilutions of artemether-lumefantrine used in agar dilution susceptibility tests.
| Artemether-lumefantrine Suspension |
–
|
| Step |
Concentration of Powdered Tablet (mg/mL) |
Source |
Volume (mL) |
Diluent (mL) |
Intermediate Concentration (mg/mL) |
Final Concentration at 1:10 in Agar (mg/mL) |
Log2 |
|
1600 |
Stock |
- |
- |
1600 |
160 |
7.32 |
| 1 |
1600 |
Stock |
2 |
2 |
800 |
80 |
6.32 |
| 2 |
1600 |
Stock |
1 |
3 |
400 |
40 |
5.32 |
| 3 |
1600 |
Stock |
1 |
7 |
200 |
20 |
4.32 |
| 4 |
200 |
Step 3 |
2 |
2 |
100 |
10 |
3.32 |
| 5 |
200 |
Step 3 |
1 |
3 |
50 |
5 |
2.32 |
| 6 |
200 |
Step 3 |
1 |
7 |
25 |
2.5 |
1.32 |
| 7 |
25 |
Step 6 |
2 |
2 |
12.5 |
1.25 |
0.32 |
| 8 |
25 |
Step 6 |
1 |
3 |
6.25 |
0.63 |
-0.67 |
| 9 |
25 |
Step 6 |
1 |
7 |
3.1 |
0.32 |
-1.69 |
| 10 |
3.125 |
Step 9 |
2 |
2 |
1.6 |
0.16 |
-2.64 |
| 11 |
3.125 |
Step 9 |
1 |
3 |
0.8 |
0.08 |
-3.64 |
| 12 |
3.125 |
Step 9 |
1 |
7 |
0.4 |
0.04 |
-4.64 |
This table is modified from Clinical and Laboratory Standard Institute . (2016) Performance Standard for antimicrobial susceptibility : 26th ed. CLSI M100S. Clinical and Laboratory Standard Institute. Wayne, PA.