Table 2: Scheme for preparing dilutions of artemether-lumefantrine used in broth dilution susceptibility tests.

Artemether-lumefantrine suspension – – – –
Step Concentration of Powdered Tablet (mg/mL) Source Volume (mL) Diluent
(mL) 		Intermediate Concentration (mg/mL) Volume (mL) Inoculum Suspension with Broth (mL) Final Concentration at 1:2 in Broth (mg/mL) Log2
1 320 Stock - - 320 0.5 0.5 160 7.32
2 320 Stock 0.5 0.5 160 0.5 0.5 80 6.32
3 320 Stock 0.5 1.5 80 0.5 0.5 40 5.32
4 320 Stock 0.5 3.5 40 0.5 0.5 20 4.32
5 40 Step 4 0.5 0.5 20 0.5 0.5 10 3.32
6 40 Step 4 0.5 1.5 10 0.5 0.5 5 2.32
7 40 Step 4 0.5 3.5 5 0.5 0.5 2.5 1.32
8 5 Step 7 0.5 0.5 2.5 0.5 0.5 1.25 0.32
9 5 Step 7 0.5 0.5 1.25 0.5 0.5 0.63 -0.67
10 5 Step 7 0.5 1.5 0.63 0.5 0.5 0.32 -1.69
11 0.63 Step 10 0.5 3.5 0.31 0.5 0.5 0.16 -2.64
12 0.63 Step 10 0.5 0.5 0.16 0.5 0.5 0.08 -3.64
13 0.63 Step 10 0.5 1.5 0.08 0.5 0.5 0.04 -4.64

This table is modified from Clinical and Laboratory Standard Institute . (2016) Performance Standard for antimicrobial susceptibility : 26th ed. CLSI M100S. Clinical and Laboratory Standard Institute. Wayne, PA.