Fig. (3) Strongly immunogenic component of ~40kD is α-haemolysin. Recombinant His-tagged α-haemolysin of S. aureus and staphylococcal anatoxin were subjected to Western blot. Membrane was treated with pre-immune serum (left panel) or mouse serum directed against recombinant α-hemolysin (right panel). Molecular mass markers are shown on the left.