Fig. (5) Biofilm formation of S. aureus O15.Panel A. Growth of S. aureus is not influenced by the added rabbit sera. S. aureus was cultivated in a 96-well plate overnight at 37°C with or without rabbit sera and optical density of a culture was measured at 560 nm.Panel B. Rabbit sera against domain B of SasG inhibits biofilm formation. S. aureus was cultivated in a 96-well plate overnight at 37°C with or without serial two-fold dilutions of rabbit sera and biofilm formation was assayed as described in Materials and Methods section. Standard deviation is shown (n=4). Blue, red and green bars represent optical density obtained with S. aureus cultures grown in the presence of non-immune, anti-absorbed staphylococcal anatoxin and anti-SasG domain B rabbit sera respectively. Grey bar denotes S. aureus culture grown without added serum.